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KMID : 0811720120160040225
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Korean Journal of Physiology & Pharmacology
2012 Volume.16 No. 4 p.225 ~ p.230
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Immunostimulatory Effects of b-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes
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Kim Ji-Mi
Joo Hong-Gu
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Abstract
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We investigated the effects of Ղ-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that Ղ-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. Ղ-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that Ղ-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. We also investigated the IL-2 dependency of Ղ-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, Ղ-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and Ղ-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that Ղ-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of Ղ-glucan in research fields.
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KEYWORD
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Ղ-glucan, Anti-apoptosis, IL-2 dependency, Splenocytes, Viability
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